proA [2017-8-11 16:2:24]

glutamate-5-semialdehyde dehydrogenase, required for normal and osmoadaptive proline biosynthesis

Locus: BSU13130

Isoelectric point: 5.10

Molecular weight: 45.17 kDa

Protein length: 415 aa

Gene length: 1245 bp

Function: biosynthesis of proline

Product: glutamate-5-semialdehyde dehydrogenase

Essential: no

E.C.: 1.2.1.41

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of proline
• Lifestyles → Coping with stress → Coping with hyper-osmotic stress

Gene

Coordinates: 1,379,605 → 1,380,852

Phenotypes of a mutant

• auxotrophic for proline PubMed

The protein

Catalyzed reaction/ biological activity

• L-glutamate 5-semialdehyde + phosphate + NADP⁺ = L-glutamyl 5-phosphate + NADPH (according to Swiss-Prot)

Protein family

• gamma-glutamyl phosphate reductase family (according to Swiss-Prot)

Structure

• 1O20 (from Thermotoga maritima, 46% identity, 67% similarity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: proB-proA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • T-box: anti-termination, in T-box
  • YlxR: regulation, PubMed, in YlxR regulon

  Regulation

  • induced by proline limitation (T-box) PubMed
  • expression requires functional YlxR PubMed

  Additional information

  • ProA is absent from the membrane proteome of a 'BdbC-BdbD' mutant PubMed
  • the T-box RNA is degraded by RNase Y PubMed

References

Reviews

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Original publications

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